Kala-azar (visceral leishmaniasis) is an infectious disease caused by a protozoan parasite, transmitted by a blood-sucking insect sandfly, smaller than mosquito and is almost lethal if untreated. Cheap, easy to perform even for a newly trained technician and yet high-performance diagnostic kits are essential for the disease control. We have developed two diagnostic methods so far. One is a sensitive method which detects antibodies to the parasite in urine samples. Urine collection is much easier and compliant than collecting blood samples; making it suitable for large-scale epidemiological studies. However, the antibodies don’t disappear readily after complete treatment and subsequently the positive results may not indicate true positives. Another is the rapid DNA amplification, LAMP method. The LAMP is more sensitive yet simple than PCR; the results can be observed with naked eyes. LAMP positives indicate the parasite positives. To contribute for kala-azar control with these tools is the major goal of our group. Only human has been considered as the reservoir of the parasite and man to man transmission by sandflies causes epidemic of kala-azar in Bangladesh. Along with PKDL cases, which develop after treatment of kala-azar, asymptomatic carriers might play certain roles as the parasite reservoirs. Although finding these parasite carriers is essential for the kala-azar control, it is not so easy to find the carriers without any symptoms. Our two step diagnostic protocol will be useful to detect this group. Improvement efforts are ongoing to make them simpler and more convenient.